摘要
目的体外研究二乙酰己二胺(CAHB)对人骨髓增生异常综合征(MDS)细胞株MUTZ-1细胞的作用以及可能的作用机制。方法用CAHB处理MUTZ-1细胞,光学显微镜下观察不同浓度的CAHB处理后MUTZ-1细胞形态的改变;利用四甲基偶氮唑盐比色法(MTT法)检测CAHB对MUTZ-1细胞的增殖抑制作用;采用流式细胞仪分析细胞DNA含量分布变化;利用RT-PCR技术检测凋亡相关基因Survivin、Bcl-2的表达变化。结果CAHB能抑制MUTZ-1细胞的生长,具有浓度和时间依赖性;随着CAHB药物浓度的增加MUTZ-1细胞出现凋亡细胞的典型形态学特征改变;CAHB作用后,MUTZ-1细胞的DNA含量在细胞周期的分布有明显变化,G0/G1期细胞比例无明显变化,S期细胞减少,而G2/M期细胞增多,细胞被阻滞在G2期,呈浓度依赖;在细胞凋亡过程中抗凋亡基因Bcl-2、Survivin基因下调。结论CAHB不但能抑制MUTZ-1细胞生长而且能诱导细胞凋亡,诱导细胞凋亡是其主要细胞毒作用之一。抗凋亡基因Bcl-2、Survivin基因下调可能是其诱导细胞凋亡的机制之一。
Objective To investigate the effect of CAHB on the apoptosis of MUTZ-1 ceils in vitro and its mechanism. Methods The morphologic features of MUTZ-1 cells were observed using light microscopy, cell growth was measure by MTT assay, cell cycle was detected by flow cytometry and mRNA expression of survivin and Bcl-2 genes was detected by RT-PCR methods. Results MTT assay showed that treatment with CAHB remarkably inhibited the growth of MUTZ-1 cells in a dose-dependent and time-dependent manners. The typical apoptotic morphological features appeared in cells treated with CAHB. Flow cytometry showed that MUTZ-1 cells had a dose-dependent apoptosis after cultured with CAHB for 24 h. CAHB treatment decreased S phase ceils, increased G2/M phase ceils, but G0/G1 phase did not changed. RT-PCR showed that survivin and Bcl-2 inhibit MUTZ-1 cell growth and induce cell apoptosis survivin genes in MUTZ-1 cells. mRNA down-regulated in MUTZ-1 cells. Conclusion CAHB can which may be associated with down-regulation of Bcl-2 and survivin genes in MUTZ-1 cells.
出处
《浙江医学》
CAS
2008年第5期466-469,共4页
Zhejiang Medical Journal
