高效毛细管电泳安培法测定海虾中呋喃唑酮的残留量

Determination of Furazolidone Residue in Shrimp by Capillary Electrophoresis with Amperometric Detection

建立了测定虾中呋喃唑酮残留量的高效毛细管电泳-安培法。探讨了缓冲溶液浓度、酸碱度及其操作电压，进样时间，有机添加剂等条件对检测的影响。在硼砂-氢氧化钠（硼砂浓度为30mmol／L，pH=9．0）为电泳介质，甲醇为有机添加剂，在25kV分离高压的实验条件下：方法的线性范围为0．5～100mg／L，相关系数r=0．9992．检出限为0.01mg／L。

A method for the determination of furazolidone residue in shrimp by high performance capillary electrophoresis with amperometric detection system was established. Several parameters affecting the determination were studied, including running buffer concentration, acidity, sampling time and separation voltage and organic additive. Under the following conditions ： Na2B4O7-NaOH（ Na2B4O7 30 mmol/L, pH =9.0 as buffer solution, CH3OH as organic additive, 25 kV as running voltage, a linearity exists between peak height（Y） and mass concentration（c） in a range of 0. 5 - 100 mg/L, the linear coefficient is 0. 999 2, determination limit is 0. 01 mg/L.