马桑内酯致痫对大鼠突触及血脑屏障超微结构的影响

Effect of Coriaria Lacton on Ultrastructure of Synapse and Blood-brain Barrier in Epilepsy Rats

目的观察马桑内酯致痫大鼠运动皮质突触和血脑屏障的超微结构改变,探讨突触和血脑屏障在癫痫发作过程的可能机制。方法成年健康雄性SD大鼠20只,随机分为对照组、癫痫组;癫痫组用马桑内酯注入到大鼠侧脑室,制作癫痫动物模型;7天后取大脑运动皮质,做超薄切片,电镜观察运动皮质突触和血脑屏障的超微结构改变。结果①癫痫组突触界面弯曲形态、突触活性区、突触后致密物和穿孔性突触明显增加;②癫痫组内皮细胞、基膜、周细胞出现明显水肿,基膜电子密度降低。结论①马桑内酯致痫改变了大鼠突触和血脑屏障超微结构,提高突触的传导活性,增强血脑屏障的通透性;②突触和血脑屏障的结构改变可能是癫痫发作重要机制。

To observe the change of the synapse and blood-brain barrier ultrastructure in the epilepsy rats motor cortex induced by coriaria lacton, and to discuss the effect of the synapse and blood-brain barrier on mechanism of the falling sickness. Method 20 health male Sprague Dawley rats were randomly divided into two groups： control group and epilepsy group. The coriaria lacton was injected into lateral ventricles of the epilepsy group rats to make the epilepsy animal model. The motor cortex was cut after 7 days, and the ultrathin sections were made, and then the change of synapse and blood-brain barrier ultrastructure were observed with electron microscope. Results ①Curvature of synapic interface, length of active zones, thickness of postsynaptic density and percentage of perforation synapse increased significantly; ②The endothelium, basement membrane, pericyte of the epilepsy group got significant edema, and electron density of basement membrane was reduced. Conclusion ①The coriaria lacton change the ultrastructure of synapse and blood-brain barrier of the epilepsy rats, and improve the transmited energy of the synapse, and enhance permeability of blood-brain barrier; ②The change of synapse and blood-brain barrier ultrastructure might be the important mechanism for epilepsy.