骨诱导因子mRNA表达与乳腺癌发生和转移的关系

mRNA level of osteoglycin correlated with carcinogenesis and metastasis of breast cancer

目的:探讨骨诱导因子(OGN)基因mRNA表达与乳腺癌发生、发展及转移的关系。方法:采用实时定量RT-PCR方法检测30例乳腺良性肿瘤、108例乳腺原发癌组织和其中30例的癌旁正常组织、30例的淋巴结转移癌中OGN mRNA的表达状态,并分析乳腺原发癌中OGN mRNA表达水平与临床病理因素的关系。结果:OGN mRNA在乳腺癌旁正常组织、良性肿瘤、原发癌和转移癌的高表达率分别为76.7%、83.3%、44.4%和6.7%,其中在非癌的良性肿瘤与正常组织间无差异,在乳腺原发癌的表达比非癌组织下调(P<0.05),在转移癌的表达比原发癌下调(P<0.001)。乳腺原发癌中OGN mRNA表达水平仅与患者年龄相关,而与肿瘤大小、临床分期、组织学分级和淋巴结状态等临床病理因素无关。结论:OGN mRNA低水平状态与乳腺癌的发生和转移相关,有望作为乳腺癌诊断和监测肿瘤进展的分子标志物。

Objective： To explore the role of osteoglycin （OGN） in the development of breast cancer and the association between OGN expression and several clinicopathologic parameters in large sample. Methods： The OGN mRNA expression levels were measured in 30 benign breast tissues, 30 normal breast tissues, 108 primary tumors, and 30 lymph node metastases using real time reverse transcription-polymerase chain reaction （RT-PCR）. The relationship between mRNA levels of OGN in primary breast cancers and clinical parameters was analyzed. Results： The rate of high level OGN mRNA was 76.7% in normal breast tissues,83.3% in benign breast tissues, 44.4% in primary tumors and 6.7% in lymph node metastases respectively. OGN mRNA was down regulated in primary cancer tissues compared with benign breast tissues and normal tissues （P〈0.05）, and also down regulated in metastases relative to primary cancer （P〈0.001）. The OGN mRNA expression in breast primary cancer was associated with patient＇s age while no relationship was found with other clinical parameters such as tumor size, clinical stage, nuclear grade and lymph node status. Conclusion： Down-regulation of OGN mRNA is involved in carcinogenesis and metastasis of breast cancer and it might be a molecular marker for the diagnosis and monitoring the progress of breast cancer.