脊髓损伤后关节软骨中TGF-β和IL-6的表达

Expression of transforming growth factor-β and interleukin-6 in articular cartilage after spinal cord injury

目的:观察脊髓损伤模型大鼠膝关节软骨组织中转化生长因子-β(TGF-β)和白细胞介素-6(IL-6)表达的变化,分析其在软骨代谢中的作用,为关节软骨的修复重建提供理论依据。方法:选择健康成年SD雌性大鼠48只,随机分为脊髓损伤组和假手术组,各24只。按改良Allen打击法建立大鼠脊髓中度损伤模型,对照组仅行T9椎板切除术。伤后分别在1、2、4周麻醉下处死,两组每个时间点处死动物均为8只。用SP法进行TGF-β、IL-6免疫组化染色及通过倒置光学显微镜及成像系统行灰度值扫描,对大鼠膝关节软骨组织中TGF-β、IL-6的表达变化进行观察,并采用SPSS12.0软件对结果进行统计分析。结果:(1)免疫组化中胞浆和胞膜部位出现红色颗粒状为阳性表达。IL-6、TGF-β主要分布在关节软骨表层。(2)脊髓损伤后第1、2、4周时IL-6的表达强度均高于对照组(181.13±7.34,193.19±8.24;178.02±5.05,190.78±8.59;175.52±2.05,192.98±8.22;P<0.05)。(3)在脊髓损伤第1周时TGF-β表达与对照组相比差异无统计学意义(P>0.05),第2、4周时表达差异有统计学意义(182.25±4.73,191.40±2.87;172.85±7.28,191.41±8.53;P<0.05)。结论:脊髓损伤大鼠关节软骨中IL-6的表达增高出现较早,而TGF-β的表达则随着脊髓神经功能的恢复而增强,二者参与关节软骨的修复重建。

Objective： T o observe the expression of transforming growth factor-β （TGF-β） and interleukin-6 （IL-6） in articular cartilage in the spinal cord injury model rat and analyze its meaning in the variation of cartilage metabolism so as to provide rationale in the clinical articular cartilage reestablishment. Methods：The total 48 healthy mature female SD rats were randomly divided into 2 groups： spinal cord injury group and control group respectively.Moderate injury models were established with modified Alien method.T9 laminectomy was performed in rats of the control group.The rats were killed at week 1,2,4 after injury.TGF-β and IL-6 were stained with immunohistochemical staining （SP） and gray scanning was conducted with inverse photon microscope and imaging system.TGF-β and IL-6 in the knee joint cartilage of rats were observed, and the result and statistics processing were analyzed with SPSS 12.0 software.Results： （1） Positive signals were expressed as red granules in the immunohistochemical staining, mainly pitched at the cellular plasm and the cellular membrane.TGF-β and IL-6 were mainly distributed in the surface layer of the articular cartilage.（2）In spinal cord injury group, the positive expression intensity of IL-6 was evidently higher than in the control group at week 1, 2 and 4 081.13＋7.34; 193.19±8.24; 178.02± 5.05; 190.78±8.59; 175.52±2.05; 192.98±8.22, P〈0.05）.（3）TGF- β expression in the spinal cord injury gro up had no remarkable difference as compared with the control group at week 1 （P〉0.05）.There wereremarkable significant difference at week 2 and 4 （182.25 ±4.73,191.40±2.87; 172.85 ±7.28,191.41 ±8.53 ; P〈0.05）. Conclusion： The expression of IL-6 possesses of earlier period in time after spinal cord injury and the expression of TGF-β strengthens with revive of spinal neural function.They are important in articular re-establishment.