摘要
β-葡聚糖是以β-1,3和β-1,4混合糖苷键连接形成的D-葡萄糖聚合物,主要存在于单子叶禾本科谷实中。有些微生物会产生降解β-葡聚糖的β-1,3-1,4-葡聚糖酶。将β-葡聚糖酶基因在食品级宿主菌中表达对动物饲料工业具有十分重要的应用价值。实验以地衣芽孢杆菌为材料,提取其总DNA,扩增其β-1,3-1,4葡聚糖酶基因序列,将该片段克隆到pMD19-T载体进行测序,并将测序结果进行BLAST比对。发现其与GenBank中的两段基因序列具有较高的同源性。
β- glueanase can effectively improve digestibility of barley - based diets. Therefore, it has been applied in animal feed additive industry. The research and application over utilization of β- glucanase as a silage additive are developing quickly. Through extensive practical application, it has been generally accepted by the animal feed additive industry. If β- glucanase can be expressed in GRAS microorganism, it can be added directly to silage without any process such as production, fermentation and purification of the enzyme. In this experiment,l β- 1,3- 1,4 glucanase in Bacillus licheniformis was amplified by PCR, and linked to vector pMD19- T. The sequencing result was analyzed through BLAST.
出处
《新疆农业科学》
CAS
CSCD
2008年第3期467-469,共3页
Xinjiang Agricultural Sciences
基金
国家973计划项目“秸秆资源生态高值化关键过程的基础研究”(2004CB719704)
国家农业科技成果转化项目“青贮饲料微生物添加剂技术成果转化”(2006GB2G400336)
新疆维吾尔自治区高技术研究与发展计划项目“微生物青黄贮菌剂的分子改良及应用”(200511107)
