地塞米松对小鼠免疫系统的抑制作用

Inhibitive effect of dexamethasone on immune system of mouse

目的研究地塞米松对小鼠免疫系统的抑制作用。方法取7—8周龄昆明种小鼠，腹腔注射地塞米松，分别于注射后5、10、15、24和36h时间点取胸腺、脾脏和肠系膜淋巴结；对照组小鼠腹腔注射生理盐水（即0h组），HE染色。结果地塞米松组胸腺中凋亡细胞和凋亡小体数量多于对照组；脾脏和淋巴结内亦见凋亡细胞，但较胸腺内少；15h时间点凋亡细胞最多。结论地塞米松可引起胸腺、脾脏和淋巴结细胞的凋亡，对免疫系统有明显的抑制作用。

Objective To observe the effect and characterisitic of dexamethasone on immune system. Methods The mice of 7-8 weeks old were injected with dexamethasone. After the injection they were subjected to execute at five time points（ 5,10,15,24,36 h） and their thymus, spleen and mesenteric lymph nodes were obtained for morphologic observation. Results Compared with control mice, obviously more apoptotic cells and apoptotic bodies were found in the thymus, spleen and lymph nodes of the mice injected with dexamethasone,but less in the spleen and lymph nodes than in thymus. Apoptotic cells were most at the 15 h point. Conclusion The results suggested that dexamethasone could bring the lymphocyte apoptosis of thymus, spleen and lymph nodes, then restrain the immunologic function.