用于煤气废水深度处理的脱酚菌的特性

Degradation characteristics of phenol-degrading bacteria used for gasification wastewater advanced treatment

从哈尔滨某气化厂生化处理系统的活性污泥中筛选出2株高效脱酚菌.经过生理生化鉴定和16SrDNA测序,鉴定2株菌分别属于芽孢杆菌属(Bacillus)和假单胞菌属(Pseudomonas).研究了温度、pH对单一高效脱酚菌和混合菌脱酚能力的影响及其菌胶团形成能力.结果表明:混合菌脱酚能力和菌胶团形成能力高于单一菌种,对温度、pH的适应性也比单一菌种强,更适合作为实施生物增强的菌剂.将混合菌固定在活性炭上,形成固定化生物活性炭(IBAC)处理煤气废水,对COD和总酚的去除率可以分别达到80%和70%以上.

Two phenol-degrading bacteria were isolated from activated sludge in biological treatment system of Harbin Gasification Factory. Physiological and biochemical tests and 16SrDNA sequencing revealed that two bacteria belong to Bacillus and Pseudomonas respectively, Effects of temperature and pH on the phenol degrading capacity of single bacterium and mixed bacteria were studied; zoogloea forming capacity of two kinds of bacteria were investigated. Compared with single bacterium, mixed bacteria have better phenol degrading capacity and zoogloea forming capacity. Mixed bacteria are more suitable for bioaugmentation than single bacterium. IBAC has been developed on the granular activated carbon by immobilization of selected and acclimated species of the mixed bacteria to treat the gasification wastewater. The removal efficiencies of COD and total phenol were over 80% and 70% respectively.