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紫杉醇对NK-92MI细胞杀伤功能及凋亡的影响 被引量：1

Effect of Paclitaxel on Killing Function and Apoptosis of NK-92MI Cells
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摘要 探讨紫杉醇对人自然杀伤细胞系NK-92MI细胞株杀伤活性及凋亡的影响。用不同浓度的紫杉醇处理NK.92MI细胞后,测定NK-92MI细胞的增殖率和对靶细胞的杀伤作用;同时检测NK·92MI细胞凋亡率及凋亡相关基因Bcl-2、Ba】【的表达。结果表明,紫杉醇可以抑制NK-92MI细胞增殖并且降低其杀伤活性,机制可能是通过诱导NK.92MI细胞的凋亡,而后者与Bcll2/Ba】【基因表达增高有关。 NK-92MI cells were treated with different concentration of paclitaxel respectively to study the effect of paclitaxel on killing activity and apoptosis of NK-92MI cells. The proliferation rate and cytotoxicity of the cells were detected after the treatment. The cell apoptosis rate and the expression of apoptosis-related genes Bcl-2 and Bax were observed. The results showed that paclitaxel could inhibit the cell proliferation and reduce its cytotoxicity that may be caused by paclitaxel-induced apoptosis of the cells. The molecular mechanism of paclitaxel-induced apoptosis of NK- 92MI cells may be associated with the increasing expression of Bax/Bcl-2.
作者 李建华 傅炜昕 侯佳宜 秦博 贾秀坤 范世光 梁再赋
机构地区 中国医科大学基础医学院病理学教研室及附属第一医院 中国医科大学实验技术中心
出处 《微生物学杂志》 CAS CSCD 2008年第2期30-35,共6页 Journal of Microbiology
基金 教育部留学回国人员启动基金(2004527)
关键词 紫杉醇 NK-92MI细胞 凋亡 BCL-2 BAX paclitaxel NK-92MI cell Bcl-2 Bax
分类号 R392.2 [医药卫生—免疫学]
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参考文献12

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二级参考文献18

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共引文献22

同被引文献8

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微生物学杂志
2008年 第2期

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