粗提和纯化的HPV16L1重组蛋白为抗原的ELISA比较

ELISA Experiment Comparison of Crude and Purified HPV16L1 Recombinant Protein as Antigen

为评价真核及原核细胞表达的HPV16L1粗提蛋白代替纯化的16L1蛋白作为ELISA检测抗原的可行性。实验分别用大肠埃希菌表达的HPV16L1纯化蛋白,表达16L1的大肠埃希菌破碎物及表达16L1的昆虫细胞破碎物作为抗原。在ELISA试验中与抗HPV16L1的单克隆抗体进行反应。结果证实3种包被抗原可以得出非常相近的OD值变化趋势。表明用表达16L1的大肠埃希菌破碎物和表达16L1的昆虫细胞破碎物可以代替纯化的HPV16L1蛋白作为抗原进行ELISA试验。

The feasibility of replacement of purified HPV（ human papillomavirus） 16LI proteins with crude ones as antigen in ELISA test was evaluated. The experiment respectively used E. coli expressed HPVI6LI purified protein, pieces of E. coli that expressed 16LI, and pieces of insect cell that expressed 16LI as antigens. Serial dilutions of anti-HPVI6LI monoclonal antibody were used in the ELISA test. The results have proved that three different coating antigens could obtain very similar OD values changing trend. These indicated that pieces of E. coli and pieces of insect cell that exoressed 16LI oroteins could reolaee ourified HPV16LI oroteins as antigen in ELISA test.