我国引种海带和栽培品种(系)来源配子体克隆的AFLP分析

AFLP Analysis of the Gametophyte Clones Derived from Introduced Laminaria (Phaeophyta) and Cultured Varieties of China

用AFLP方法进行了11个海带自然种群和养殖品种(系)来源的配子体克隆的遗传分析。9对引物组合共扩增出246个位点,仅14个位点为单态,多态比例达到94.3%。各材料间的Nei’s遗传距离在0.280～0.726之间,平均为0.512,其中长海带与其它材料间的遗传距离最远。UPGMA聚类分析将各材料大致分为3类群,基本反映了各材料来源孢子体表型特征的分化,也在一定程度上反映了目前海带养殖品种间存在混杂。将扩增图谱转换成[0/1]型数据集,构建了不同材料的指纹图谱,发现55个特征标记,其中26个为特异标记。

Eleven gametophyte clones of introduced Laminaria species and cultured varieties of China were analyzed using amplified fragment length polymorphism（AFLP）. In total, 246 loci were obtained using 9 combinations of selective primers. Of these loci, 231 were polymorphic, accounting for 94.3% of the total. The genetic distances between gametophyte clones ranged from 0. 280 to 0. 726. Genetic distances between L. longissima and other materials were the longest. This was consistent with its origin. Phenotypic differentiation of these materials was demonstrated clearly in neighbor-joining tree constructed based on genetic distances. In addition, fingerprints consisting of 55 bands of 11 materials were obtained by conversing AFLP patterns into a [0, 1 ] data matrix, of them, 26 were specific for different materials. Currently, we are trying to convert these bands into sequence tagged sites, which should facilitate the identification of Laminaria species and varieties and Laminaria genetic improvement.