黄连、土大黄、苍术提取液抗银屑病实验研究

Experimental Study of Three Kinds of Chinese Herbs in Treating Psoriasis

目的探讨中药黄连、土大黄、苍术提取液抗银屑病的作用机制。方法采用体外细胞培养技术,选用人角质形成细胞株colo-16,观察黄连、土大黄、苍术单方及复方中药提取液对肿瘤坏死因子-α(TNF-α)刺激前后人角质形成细胞增殖的影响。结果黄连、土大黄、苍术单方及复方中药提取液在TNF-α刺激前后均表现出显著的抑制人角质形成细胞增殖的作用,与对照组甲氨喋呤和环孢素A相比较,差异有显著性意义(P<0.01)。结论中药复方通过抑制人角质形成细胞的异常增殖对银屑病有治疗作用。

Objective To study the mechanism of the extract of three kinds of Chinese herbs in treating psoriasis. Methods Three kinds of Chinese herbs （coptis root, patience dock root and atratylodes rhizome） were extracted. With the culture of human transformed keratinocyte cell line colo-16 cells as a model, the single and compound effects on the growth of KC were studied before and after stimulated by TNF-α. Results The single and compound extract of three Chinese herbs could restrain the proliferation of KC before and after stimulated by TNF-α. The effect was more remarkable than the compared methotrexate and cyclosporin A （P〈0.01）. Conclusions The three kinds of Chinese herbs can treat psoriasis by restraining the proliferation of KC.