电针足三里对吗啡戒断大鼠μ受体mRNA表达的影响

Effects of Electroacupuncture on Expression of μ-opioid Receptor mRNA of Morphine Withdrawal Rat

目的探讨电针改善吗啡戒断症状的作用机制。方法建立自然吗啡戒断大鼠模型,运用RT-PCR方法测定脑组织μ受体mRNA的表达,观察电针足三里对吗啡戒断大鼠体重及μ受体mRNA表达的影响。结果电针组大鼠体重比戒断组增加明显(P<0.05),戒断组和电针组比正常组脑组织中μ受体mRNA表达降低,治疗后电针组比戒断组脑组织中μ受体mRNA表达明显增加。结论电针足三里具有提高吗啡戒断大鼠体重、改善大鼠吗啡戒断症状的作用。电针调节吗啡戒断大鼠海马、下丘脑μ受体mRNA的表达,可能是电针改善大鼠吗啡戒断症状的作用机制之一。

Objective To research the mechanism of electroacupuncture （EA） improving morphine abstinence syndrome in rats. Methods The model of the rat with physical morphine abstinence syndrome was established. The expression of μ-opioid receptor mRNA in the brain tissue of morphine withdrawal rats was examined by RT-PCR, and effects of EA at Zusanli （ST36） on the body weight and expression of μ-opioid receptor mRNA of morphine withdrawal rat were observed. Results The body weight of the EA group was increased compared with the morphine abstinence group （P 〈0.05）. Comparing with the control group, the expression of μ-opioid receptor mRNA in the brain was lower in the EA group and the morphine abstinence group. After EA, the expression of μ-opioid receptor mRNA of the EA group was more increased than the morphine abstinence group. Conclusion EA at Zusanli （ST-36） can increase body weight of morphine withdrawal rats, and improve morphine abstinence syndrome. EA can increase expression of μ-opioid receptor mRNA in hippolcapal and hypothalamus of brain tissue in morphine withdrawal rat, that may be one of the mechanisms of EA improving morphine abstinence syndrome of rats.