HPLC法测定牛黄清胃丸中大黄素和大黄酚的含量

Determination of Emodin and Chrysophanol in Niuhuang Qingwei Pills by HPLC

目的建立HPLC测定牛黄清胃丸中大黄素和大黄酚含量的方法。方法采用HPLC法,Hypersil C18色谱柱(5μm,4.0 mm×250 mm);流动相:甲醇-0.1%磷酸溶液(85∶15);流速1.0 mL/min;检测波长254 nm;柱温30℃;进样量10μL。结果大黄素、大黄酚均在0.04～1.60μg范围内呈良好的线性关系,r=0.9999,平均回收率为99.92%,RSD=0.6%。结论所建立的方法简便可行,重复性好,可作为牛黄清胃丸的质量控制方法。

Objective To establish a quality standard of Niuhuang Qingwei Pills. Methods Hypersil C18 was used with methanol-0.1% Phosphoric acid as mobile phase. The flow rate was 1.0 mL/min, detective wavelength was 254 run, column temperature was 30℃ and injection valume was 10 μL. Results Emodin and chrysophanol showed a good linear relationship in the range of 0.04-1.60 μg （r=0.999 9）. The average recovery was 99.92% （RSD=0.6%）. Conclusion The established method is simple, feasible and reproducible, and can be used for the quality control of Niuhuang Qingwei Pills.