杭州市2007年流行性腮腺炎病毒分离株的基因型分析

Analysis of genotypes of mumps virus in Hangzhou in 2007

目的:了解杭州地区腮腺炎病毒流行株基因型分布情况。方法:对杭州地区2007年腮腺炎病毒分离株SH基因测序后分型,测序结果与其他基因型参考株进行同源性比较,构建SH基因亲缘进化树。结果:杭州地区2007分离株病毒属于F基因型,与浙江株EF102876、EF102880相似性分别为99.3%、96.7%;与基因型代表株Z77158(兰州)、Z77160(上海)核苷酸相似性分别为98%、94.6%;与目前使用的疫苗株D90232(Jeryl Lynn)相似性为84.9%。结论:从本次分离株来看,杭州地区流行的为F基因型,未发现基因型间变异,但与上海株之间存在较大的型内差异。

Objective：To study the genotypes of mumps virus strains in Hangzhou in 2007.Methods：Genotypes of mumps virus strains were determined by nucleotide sequence analysis to the small hydrophobic（SH） protein gene in Hangzhou in 2007.The phylogenetic tree of the SH gene was established by comparative analysis of the nucleotide sequence.Results：Genotypes of mumps virus strains was F type,whose homology with two Zhejiang isolates（EF102876、EF102880） was 99.3% and 94.6% respectively.Compared with other three representative isolates,Z77158（Lanzhou）,Z77160（Shanghai）and D90232（Jeryl Lynn）,the homology was 99.3%,94.6% and 84.9% respectively.Conclusion：Genotypes F of mumps virus strains from Hangzhou does not exist genotypes variance,but some difference existed in nucleotide sequence with comparison to Z77160（Shanghai）.