分光光度法快速测定尿中百草枯

Determination of paraquat in urine with spectrometry method

目的：为尿中百草枯的测定研究一种简单可靠的测定方法,从而为临床诊断与治疗提供依据。方法：于尿样中加入固体碳酸钠和碳酸氢钠调pH,慢速定量滤纸过滤,加入连二亚硫酸钠显色,于396 nm比色测定。结果：百草枯在0-4.0μg/ml呈良好线性,相关系数为0.9991,回归直线方程Y=0.15408X-0.00267。最低检出限为0.15μg/ml。高中低浓度的百草枯溶液精密度分别为5.2%、2.1%、4.5%,回收率96.5%-103.0%。结论：此方法操作简便,灵敏度较高,完全能满足尿中百草枯检测的需要。

Objective：It is a reliable and exact method to analyze and study the paraquat in urine.Methods：Take Na2CO3 and NaHCO3 to the urine to make pH〉9,and be filtered with film,take Na2S2O4,and with spectrometry to detect in 396 nm.Results：It has a good linearity when the contents of paraquat in 0-4 μg/ml,and the relevant coefficient are 0.9991.The limits of detection were 0.15 μg/ml.The variable coefficients are 5.2%,2.1%,4.5%,with average recovery rate between 98.8%-101.5% at different spiking level.Conclusion：It is proved that this method is simple and fast and with high sensitivity.And it can meet the need of detection paraquat in urine entirely.