两种检测乙肝病毒前S区抗原方法的比较

Comparison of two methods detecting pre-S antigen of hepatitis B virus

目的:检测病人血清中乙肝病毒表面抗原大蛋白(LHBs)和乙肝病毒前S1蛋白(Pre-S1),与HBV DNA比较,探讨LHBs和Pre-S1检测方法的可靠性和实用性。方法:采用荧光定量PCR方法检测HBV病人血清的HBV DNA;采用酶联免疫吸附试验(ELISA)的方法检测HBV病人血清中的LHBs和Pre-S1。结果:124份HBV病人血清中LHBs检出阳性108份,阳性率87.10%;pre-S1检出阳性61份,阳性率49.19%,两者有统计学意义差异(P<0.05)。LHBs与HBV DNA检测结果显示良好的相关性。结论:检测LHBs比检测Pre-S1更能反映病人体内HBV复制情况,LHBs的检出与HBV DNA拷贝数具有较好的相关性。

Objective：To study the reliability and practicability by comparing the method of detecting hepatitis B Virus large envelope protein（LHBs） and pre-S1 with the method of detecting HBV DNA in serum.Methods：HBV DNA in patient serum was detected with fluorescent quantitative PCR.LHBs and pre-S1 in patient serum were detected with ELISA as well.Results：A hundred and eight samples were tested LHBs positive from 124 patient serum samples（positive ratio=87.10%） while 61 positive cases of pre-S1 were identified（positive ratio=49.19%）.There was statistical significance（P〈0.05）.A good correlation was found between test results of LHBs and HBV DNA.Conclusion：The copying process of HBV in patients can be better understood to detect LHBs than to detect pre-S1.A good correlation is observed between the detection of LHBs and the copies of HBV DNA.