胶体金免疫层析法应用于感染性肠道疾病病原的快速检测

Application of gold colloid immunochromatography for rapid detection of pathogens in infectious enteric diseases

目的:寻求能够应用于感染性肠道疾病现场,快速检测病原体的方法。方法:应用胶体金免疫层析法(GICA)检测出血性大肠杆菌O157:H7、单增李斯特菌、金黄色葡萄球菌肠毒素、肠炎沙门菌、霍乱弧菌小川型、霍乱弧菌O139群6种菌株模拟标本和流行期的霍乱监测标本,并与培养法相比较,以评价方法的敏感性、特异度和符合率。结果:上述6种细菌检测卡的检测限分别是106、106、108、108、107、107cfu/ml。除O1群霍乱弧菌检测卡与非O1/O139群霍乱弧菌VBO12型有弱阳性反应外,各检测试剂卡与非检测目标菌液呈阴性反应。实际环境样品检测结果敏感性为50%,特异度为99%,总符合率98%,一致性较好。结论:胶体金快速诊断技术与其他方法相比,在许多方面具有无可比拟的优势,但其敏感性有待提高。

Objective：To seek a rapid detection method for pathogens of the infectious enteric diseases in the scene.Methods：The gold colloid immunochromatography（GICA） was tested using six imitated samples contained Escherichia coli O157：H7（O157）,Listeria monocytogenes,enteric toxin of Staphylococcus aureus,Salmonella enteritidis,Vibrio cholerae of Ogawa,Vibrio cholerae O139 group and the samples of cholerae surveillance collected from epidemicperiod,then compared with culture method in order to evaluate the sensitivity,specificity and coherence of GICA.Results：The minimal detection limits of the six GICA cards were 10^6,10^6,10^8,10^8,10^7,10^7 cfu/ml.The GICA card showed a negative result after the test using the non-target bacterium liquid except Vibrio cholerae O139 group card and Vibrio cholerae non-O1/O139 group showing weak positive results.The sensitivity of the sample results was 50%,the specificity 99% and the coherence also good.Conclusion：The GICA has many advantages compared with the other methods except that its sensitivity should be improved.