Ⅱ型糖尿病患者单核细胞表面CD36表达和单核细胞-血小板聚集的临床研究

Clinic study of moncytes CD36 expression and monocyte-p1atelet aggregates in type 2 diabetic patients

目的:探讨Ⅱ型糖尿病有无血管病变、患者外周血单核细胞CD36表达和单核细胞-血小板聚集的临床意义。方法:采用流式细胞仪,测定56例糖尿病伴血管病变患者及53例无血管病变患者外周血单核细胞表面CD36平均荧光强度和单核细胞-血小板聚集的百分率,并与56例健康对照组比较。结果:糖尿病伴血管病变患者外周血单核细胞CD36平均荧光强度和单核细胞-血小板聚集的百分率均明显高于无血管病变组和健康对照组(P<0.01);且伴血管病变组外周血单核细胞CD36平均荧光强度与单核细胞-血小板聚集的百分率具有显著的正相关性(r=0.582,P<0.01)。结论:糖尿病患者长期慢性高血糖产生糖基化产物(Advanced Glycation End-product,AGE),可能会促使CD36表达增加,进一步触发单核细胞-血小板聚集体增加,从而加速动脉粥样硬化的发生和发展。

Objective：To study the monocyte CD36 expression and monocyte-platelet aggregates（MPAs） in the type 2 diabetic patients.Methods：The monocyte CD36 expression（Mean fluorescence intensity,MFI） and MPAs percentage were measured in 56 diabetic patients with vascular complications and 53 diabetic patients without vascular complications and control group by FCM.Results：Mean monocyte CD36 expression MFI was higher in the patients with vascular complications（46.2±4.17） than those without vascular complications（22.7±4.65） and control group（27.5±3.01）（P〈0.01）.The MPAs formations were also higher in the patients with vascular complications（57.4±12.9%） than without vascular complications（19.6±8.7%） and control group（14.3±6.6%）（P〈0.01）.Furthermore,a significant correlation between CD36 MFI and MPAs percentage levels was observed in diabetic patients with vascular complications.Conclusions：The enhanced monocyte CD36 expression and increased MPAs formations play an important role in the development of diabetic angiopatny.They may provide an auto-feed mechanism for accelerating the atherosclerosis in diabetics.