共振瑞利散射法测定多柔比星脂质体中游离多柔比星的含量

Resonance rayleigh scattering method for the determination of free doxorubicin content in doxorubicin liposome

目的:建立共振瑞利散射法(RRS)测定多柔比星(DOX)脂质体中游离 DOX 含量。方法:在 pH 2.6的 Britton-Robinson(BR)缓冲液中,刚果红(CR)与 DOX 通过分子间作用力形成离子缔合物,在λ_(ex)=λ_(em)=380 nm 波长时能使 RRS 信号显著增强。结果:RRS 法在1～12 μg·mL^(-1)范围内呈线性关系,检出限为0.05^(-1)g·mL^(-1)。对6个不同批号的 DOX 脂质体混悬液中游离 DOX 的含量测定,结果与紫外可见分光光度法相符,RSD(n=6)为1.9%～3.2%,平均回收率为94.3%。结论:此方法灵敏度较高,可以用于测定 DOX 脂质体中游离 DOX 的含量。

Objective ：To develop resonance rayleigh scattering（RRS） method for the determination of free doxorubicin （DOX） content in DOX liposome. Method： In Britton - Robinson buffer of pH 2.6, congo red （CR） was combined with DOX by intermolecular forces to form complexes, causing an enhance of RRS at wavelength of about 380 nm. Based on the enhancement of RRS, a novel method can be applied to the determination of DOX. Result： The linear range for the determination of DOX was 1 - 12 μg·mL^-1 and the determination limits for DOX was 0. 05μg·mL^-1. Among six times determination, the result of the determination of free DOX according with ultraviolet spectrophotometry,the RSD was 1.9% -3.2% and the average recovery was 94.3%. Conclusion：The method is highly sensitive. The method is applied to the determination of free DOX content in DOX liposome.