菝葜组织培养研究

Study on the Technology of Tissue Culture on Smilax china L.

[目的]更好地开发利用菝葜。[方法]以菝葜幼嫩茎段为外植体,以MS1、/2 MS1、/4 MS为基本培养基,研究不同植物激素组合对菝葜组织培养快速繁殖的影响。[结果]外植体的最佳消毒方式为0.1%升汞消毒9 min。1/2 MS1、/4 MS培养基更有利于菝葜芽的诱导、增殖及生根,6-BA对芽丛的增殖培养具有关键作用。最佳启动培养基为1/2 MS+6-BA 2.0 mg/L+IBA 0.1 mg/L+NAA 0.2 mg/L。最佳增殖培养基为1/2 MS+6-BA 3.0 mg/L+IBA 0.2 mg/L+NAA 0.2 mg/L。最佳生根培养基为1/4 MS+IBA 0.1 mg/L+NAA 1.5 mg/L+活性炭1 000 mg/L,生根率最高,达75%。[结论]该研究建立了菝葜的组织培养繁殖体系,增加了菝葜的繁殖系数。

[Objective] The aimofthe researehwas to develop and utilize Smilaxchina L. better. [Method] With tender stems ofS. china as explants, MS, 1/2 MS and 1/4 MS were taken as basic medium to study the effects of different plant hormone eomhinations on the tissue culture and rapid propagation of S. china, [ Result] The optimum disinfection method of explants was disinfecting with 0.1% mereuric chloride for 9 min, Medium 1/2 MS and 1/4 MS were more favorable for the bud induction, prtrpagation and rooting of S. china. 6-BA played a key effect on the propagation culture of bud clumping, The optimum initial medium was 1/2 MS ＋ 6-BA 2.0 mg/L ＋ IBA 0.1 mg/L ＋ NAA 0.2 mg/L. The optimum propagation medium was 1/2 MS ＋ 6-BA 3.0 mg/L ＋ IBA 0.2 mg/L ＋ NAA 0.2 mg/L. The optimum rooting medium was 1/4 MS ＋ IBA 0.1 mg/L ＋ NAA 1.5 mg/L ＋ activated carbon 1 000 mg/L, with the rooting rate being highest （75%）. [ Conclusion] The tissue cuhure and propagation system was established and the propagation coefficient of S. china was increased in this research.