小麦脱水应答转录因子(TaDREBs)的序列分析及TaDREBs基因在部分小麦品种的分布

Sequence Analysis of the Known Dehydration Response Transcription Factor TaDREBs in Wheat and Its Distribution in Different Wheat varieties

[目的]了解TaDREBs 基因之间的进化关系以及TaDREBs 在小麦中的存在情况。[方法]用4 种引物TaDREBs （ TaDREB1 ,2 ,3 ,5） 在 35 种小麦品种中进行扩增, 用PowerBlast1 .2 .0 等4 个分析软件对小麦脱水应答转录因子TaDREBs（TaDREB1 ,2 ,3 ,5 ,6） 的序列进行比较, 并建立进化树。[结果]各个TaDREBs 基因之间的同源性有较大的差异,TaDREB1与TaDREB6的同源性高达87% , 其余各转录因子的同源性在44%～53%之间, 表明这类基因在干旱应答中所起的作用并不相同。在所有供试小麦品种中扩增到TaDREB5 基因, 扩增条带在 298 bp 左右, 而其他几个TaDREBs 基因在少数小麦品种没有扩增到。[结论]TaDREB5 基因可能是小麦耐旱相关重要的基因, 它的多态性可能对揭示小麦抗旱机理有重要作用。

The research aimed to understand the evolutionary relationships among TaDRF, Bs genes and the existing stations of TaDRF, Bs in wheat. [Method] Four kinds of primers TaDREBs（TaDREB1,2,3,5）were used to make amplification in 35 wheat species. The sequences of dehydration response transcription factor TaDREBs （TaDREB 1,2,3,5,6 ）were compared by using 4 analysis softwares such as PowerBLast1.2,0. [ Result ] The homologies among each TaDREBs genes had greater difference. The homology between TaDREB 1 and TaDREB6 reached 87 %, while that among other transcription factors were in 44% - 53%, which indicated that the role of this type of genes in drought response were different. TaDREB5 gene was amplified in all the tested wheat varieties with the amplification band of about 298 10p. While oher several TaDREBs genes were not amplified in few wheat varieties, [ Conclusion] TaDREB5 was probably an important gene related with drought resistance of wheat. Its polymorphism probably paid an important role for revealing the drought-resistance mechanism in wheat.