高效液相色谱法同时测定藿香正气水中甘草酸、欧前胡素、异欧前胡素、厚朴酚、和厚朴酚的含量

Simultaneous HPLC determination of five components in Tincture Huoxiang Zhengqi

目的:建立高效液相色谱法测定藿香正气水中甘草酸、欧前胡素、异欧前胡素、厚朴酚、和厚朴酚的含量。方法:采用C_(18)色谱柱(250 mm×4.6 mm,5 μm),以0.05%甲酸水溶液(A)-乙腈(B)为流动相,梯度洗脱(10 min,40%B;25 min,45%B;30 min,50%B;60 min,55%B),流速1.0 mL·min^(-1),检测波长254 nm。结果:甘草酸、欧前胡素、异欧前胡素、厚朴酚、和厚朴酚进样量分别在0.094～0.470,0.041～0.205,0.018～0.090,0.235～1.175,0.311～1.555μg范围内与峰面积呈良好的线性关系(r≥0.9998);平均同收率(n=6)分别为98.3%,99.6%,98.6%,98.0%,99.6%。结论:方法准确、灵敏,专属性强,为藿香正气水提供了多指标含量测定的质量控制方法。

Objective： To develop an HPLC method for the determination of glycyrrhizic acid, imperatorin, isoimperatorin, magnolol, and honokiol in Tincture Huoxiang Zhengqi. Methods： The method was conducted on an Agilent Zorbax XDB - C18 column （ 250 mm × 4. 6 mm, 5 μm ） with 0. 05% formic acid （ A ） - acetonitrile （ B ） as mobile phase（ gradient elution ： 10 min, 40% B ; 25 min, 45 % B ; 30 min, 50% B ; 60 min, 55 % B）, flow rate 1.0 mL · min^-1, detecting at 254 nm. Results： The linearity ranges of glycyrrhizic acid, imperatorin, isoimperatorin, magnolol and honokiol were 0. 094 -0. 470,0. 041 -0. 205,0. 018 -0. 090,0. 235 - 1. 175 ,and 0. 311 - 1. 555 μg,respectively ; r ≥ 0. 9998. The average recoveries （ n = 6 ） of them were 98.3 % , 99.6 % , 98.6 % , 98.0% , and 99.6 % , respectively. Conclusion：The method is accurate, sensitive and specific for quality control of Tincture Huoxiang Zhengqi.