摘要
目的:建立高效液相色谱法测定杞菊地黄丸中马钱苷的含量。方法:采用 Diamonsil C_(18)柱(250 mm×4.6 mm,5 μm),以乙腈-甲醇-0.1%磷酸溶液(9:5:86)为流动相,流速为1.0 mL·min^(-1),检测波长为236 nm。结果:马钱苷进样量在0.08~0.88μg范围内与峰面积呈良好的线性关系(r=0.9999);平均回收率(n=6)为98.8%,RSD 为0.94%。结论:该方法准确,重复性好,可用于该药的质量控制。
Objective:To develop an HPLC method for determination of loganin in Qijudihuang pills. Methods:A Diamonsil C18 column (250 mm × 4. 6 mm ,5 μm)was adoped. The mobile phase was acetonitrile -methanol -0. 1% phosphoric acid solution(9: 5: 86) ,at the flow rate of 1.0 mL · min^-1 and detecting wavelength was 236 nm. Results:The linearity was obtained within the range of 0. 08 -0. 88μg( r = 0. 9999) ;The average recovery( n = 6)was 98.8% , and RSD was 0. 94%. Conclusion : The method is convenient, accurate, reproducible, and can be used for quality control of the preparation.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第5期785-787,共3页
Chinese Journal of Pharmaceutical Analysis