利用SRAP标记研究四川高原青稞育成品种的遗传多样性

Genetic diversity analysis of the developed qingke (hulless barley) varieties from the plateau regions of Sichuan Province in China revealed by SRAP markers

利用SRAP(Sequence-related Amplified Polymorphism)分子标记技术,对25份来自四川高原的青稞育成品种进行了遗传多样性研究。结果表明:64对引物组合共检测出999条清晰条带,62对可以获得多态性条带,多态性引物组合占96.9%,共产生225条多态性条带,占总条带数的22.5%。64对引物组合共扩增出333种等位变异,平均每个引物组合检测到5.20种等位变异。遗传多样性在0(me9/em14,me9/em15)～0.8928(me6/em18)之间,平均为0.5126。聚类分析结果表明,25份材料可分成A、B、C3大类,材料聚类与其来源地有明显的相关性。25份材料间的平均遗传距离较小(0.3240),平均遗传多样性较低(0.5126),遗传基础较为狭窄。

Genetic diversity of 25 accessions of Qingke （hulless barley） varieties from the plateau regions of Sichuan Province, China, was analyzed by using SRAP （Sequence-related Amplified Polymorphism） markers. The results showed that 64 pairs of primer combinations produced 999 clear bands, of which 62 primer pairs （96.9%） amplified 225 polymorphic bands （22.5%）. Three hundred and thirty three allelic phenotypes were amplified with an average of 5.20 alleles/primer pair. The genetic diversity ranged from 0 （me9/eml4, me9/eml5） to 0.8928 （me6/eml8） with an average of 0.5126. The 25 accessions were classified into three maior grouns： A. B. and C bv cluster analysis using IIPGMA which showed sionificant relationship with the origin regions of accessions. Thus, it was suggested that the Sichuan hulless barleys could be used as elite germplasms to enhance the genetic background for super-hulless barley breeding.