Galectin-3在食管鳞癌中的表达及其临床意义

Association of galectin-3 expression with biological behaviors of squamous carcinoma of esophagus

目的研究Galectin-3在食管鳞癌中的表达情况,初步探讨其与食管鳞癌临床病理特征的关系。方法①用S-P免疫组化法检测80例食管鳞癌石蜡标本中Galectin-3蛋白的表达情况;②用RT-PCR法检测分析Galectin-3mRNA在36例胃镜下切取的新鲜食管鳞癌组织中的表达情况。结果①Galectin-3蛋白在食管癌组织、正常食管组织中的表达具有明显差异(P<0.05);在分化程度低、浸润程度深及发生淋巴结转移的食管鳞癌组织中的表达明显高于分化程度高、浸润程度浅及未发生淋巴结转移的食管癌组织(P<0.05)。②Galectin-3mRNA阳性表达率在食管鳞癌组织中为77.8%(28/36),在远癌正常组织中为13.9%(5/36),结果具有显著性差异(P<0.05);并且在分化程度低、浸润程度深及发生淋巴结转移的食管鳞癌组织中的表达明显高于分化程度高、浸润程度浅及未发生淋巴转移的组织,差异亦有显著性(P<0.05)。结论Galectin-3的差异表达,可能与食管鳞癌的发生、发展及转移有关。

Objective To investigate analyze its relationship with clinicopathological galectin-3 expression in squamous carcinioma of esophagus and characteristics. Methods S-P immunohistochemistry was used to detect the expression of galectin-3 in 80 paraffin-embedded specimens of esophageal carcinoma and 22 specimens of normal tissues adjacent to the benigh lesions of eso mRNA expression in 36 pairs of fresh squamous carcinoma phagus.RT-PCR was performed to detect galectin-3 tissues and corresponding adjacent normal mucosa. Results Out of 80 specimens of squamous carcinoma of esophagus, 62 specimens had positive expression of galectin-3, with the positive rate of 77.5 % （ 62/80 ） that was significantly higher than 27.27 % in normal esophageal tissues （6/22, P 〈 0.05 ）. The expression of galectin-3 was related to pathological grading, infiltration and lymphatic node metastasis （ P 〈 0.05 ）. The positive rate of galectin-3 mRNA expression in esophageal carcinoma was 77.8% , while that of the corresponding adjacent normal tissues was 13.9% （P 〈 0.05 ）. The high expression of galectin-3 mRNA was correlated with poor differentiation, deep invasion and lymph node metastasis of esophageal carcinoma, but not with the patients＇ age, gender and tumor size. Conclusion Galectin-3 olavs a role in the genesis, development and metastasis of esophageal squamous carcinoma.