甲基莲心碱对长春新碱抑制人胃癌细胞增殖作用的影响

Effects of neferine on gastric carcinoma cell proliferation inhibited by vincristine

目的:观察甲基莲心碱(Nef)在体外对长春新碱抑制人胃癌细胞增殖及诱导人胃癌细胞凋亡的影响。方法:采用MTT比色法、软琼脂克隆形成法检测药物的细胞毒性作用,并用流式细胞术、AO/EB荧光双染色法测定Nef对长春新碱诱导人胃癌细胞凋亡的影响。结果:2.5、5、10μmol/LNef能增强长春新碱对人胃癌细胞(SGC7901)增殖的抑制作用;10μmol/LNef能增强长春新碱(0.1、0.5、2、4μg/ml)诱导SGC7901细胞凋亡。结论:甲基莲心碱能增强长春新碱抑制人胃癌细胞增殖及诱导人胃癌细胞凋亡,为一种低毒高效的化疗增敏剂。

Objective：To study the effects of neferine on gastric carcinoma cell proliferation inhibited and induced apoptosis by vincristine in vitro.Methods：Cytotoxicity assay was tested by MTT and soft agar colony forming assay.The influence of neferine to affect vincristine to induce gastric carcinoma apoptosis was detected by PI staining flow cytometry and AO/EB double fluorescence stain.Results：2.5,5,10μmol/L Nef enhanced vincristine to inhibit the proliferation of SGC7901 cells;10 μmol/L Nef enhanced vincristine（0.1,0.5,2,4μg/ml）to induce SGC7901 cells apoptosis.Conclusion：Nef can enhance vincristine to inhibit the proliferation of gastric carcinoma and induce gastric carcinoma cells apoptosis.It is a kind of low poisonous and high effective chemosensitizers.