多肿瘤标志物蛋白芯片对泌尿系肿瘤和男性生殖系肿瘤诊断的临床价值

The clinical significance of multiple tumor marker protein chip in diagnosis of urinary system tumor and male genital system tumor

目的:探讨多肿瘤标志物蛋白质芯片在泌尿系肿瘤和男性生殖系肿瘤诊断中的临床应用价值,并建立12项肿瘤标志物联合诊断泌尿系和男性生殖系肿瘤的函数。方法:采用C-12多肿瘤标志物蛋白质芯片检测57例泌尿系肿瘤患者、38例男性生殖系肿瘤患者、11例泌尿系良性病变患者、25例男性生殖系良性病变患者,以及1203例正常体检者。所有患者均经病理学、影像学或临床确诊。结果:用C-12芯片检测泌尿系肿瘤的灵敏度是49.12%,特异度是81.81%,阳性预测值是93.33%,阴性预测值是23.68%;男性生殖系肿瘤的灵敏度是62.16%,特异度是48.00%,阳性预测值是64.86%,阴性预测值是46.15%。除前列腺癌外,C-12芯片联合检测的阳性率在泌尿系肿瘤和其他男性生殖系肿瘤中显著高于单项肿瘤标志物的检测(P<0.05)。建立的诊断判别函数对肾癌、膀胱输尿管肿瘤、前列腺癌、睾丸癌的诊断准确率分别为81.3%、94.5%、94.0%、91.0%,显著高于单项标志物检测(P<0.01)。结论:除前列腺癌外,用多肿瘤标志物蛋白质芯片检测泌尿系和男性生殖系肿瘤可提高诊断的灵敏度,优于单项肿瘤标志物;诊断判别函数可明显提高泌尿系及男性生殖系肿瘤诊断的准确率,具有一定的临床参考价值。

Objective：To evaluate the diagnosis value of multiple tumor marker protein chip in diagnosis for urinary system tumor and male genital system tumor,and set up the diagnostic function of urinary system tumor and male genital system tumor with 12 tumor markers.Methods：The serum levels of 12 tumor makers were measured in 57 urinary system tumor patients,38 male genital system tumor patients,11 urinary system benign diseases patients,25 male genital system benign diseases patients and 1203 health examinations.All the patients were definitely diagnosed by pathological,imaging and clinical diagnosis.Results：In urinary system tumor,specificity was 81.81%,sensitivity was 49.12%,positive predict value was 93.33% and negative predict value was 23.68%;in male genital system tumor,specificity was 48%,sensitivity was 62.16%,positive predict value was 64.86% and negative predict value was 46.15%.Except for prostatic carcinoma,the combining diagnosis of multiple serum tumor markers using C-12 protein chip had a great significance with diagnosis of the single serum tumor marker in both urinary system tumor and male genital system tumor（P〈0.05）.We also established the diagnostic function of urinary system tumor and male genital system tumor with 12 tumor markers.The accuracy rate of the diagnostic functions,which had a great significance with diagnosis of the single serum tumor marker（P〈0.01）,was 81.3% in renal carcinoma,94.5% in bladder and renal duct tumor,94% in prostatic carcinoma and 91% in carcinoma of testis.Conclusion：The combining diagnosis of multiple serum tumor markers using multiple tumor marker protein chip detection system,which is significantly better than the single serum tumor marker,can increase the diagnostic sensitivity for urinary system tumor and male genital system tumor except for prostatic carcinoma.And the diagnostic functions which can notably increase the accuracy rate for urinary system tumor and male genital system tumor are helpful in clinic.