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在组织芯片上利用量子点技术进行抗原的检测 被引量:8

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摘要 在组织培养和组织切片上,可利用放射性和非放射性方法检测抗原的定位。而在传统的免疫荧光分析技术中,有机荧光基团存在激发波长范围很窄、发光时间短,容易猝灭等缺点,限制了它们在蛋白定量上的使用。荧光半导体纳米颗粒量子点(quantum dots)提供了一种潜在的方法去克服有机荧光基团的这些缺陷,开始在免疫荧光分析中初步应用,并取得了令人满意的效果。本文就量子点的特性及其在肺癌组织芯片上进行不同抗原的检测并评价其效果进行了相关研究.
出处 《中华病理学杂志》 CAS CSCD 北大核心 2008年第6期416-417,共2页 Chinese Journal of Pathology
基金 国家自然科学基金资助项目(30500226)
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参考文献8

  • 1Chan WC, Nie S. Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science, 1998, 281 (5385) :2016-2018.
  • 2Seydel C. Quantum dots get wet. Science, 2003, 300(5616) :80- 81.
  • 3Sun BQ, Xie WZ, Yi GS, et al. Microminiaturized immunoassays using quantum dots as fluorescent label by laser confocal scanning fluorescence detection. J Immune Methods, 2001, 249 ( 1-2 ):85-89.
  • 4Mason JN, Tomlinson ID, Rosenthal SJ, et al. Labeling cell-surface proteins via antibody quantum dot streptavidin conjugates. Methods Mol Biol, 2005, 303:35-50.
  • 5Chan P, Yuen T, Ruf F, et al. Method for multiplex cellular detection of mRNAs using quantum dot fluorescent in situ hybridization. Nucleic Acids Res, 2005, 33 (18) :161-168.
  • 6Jaiswal JK, Mattoussi H, Mauro JM, et al. Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nat Biotech, 2003, 21 (1) :47-51.
  • 7Garon EB, Marcu L, Luong Q, et al. Quantum dot labeling and tracking of human leukemic, bone marrow and cord blood cells. Leuk Res, 2007,31 (5) :643-651.
  • 8Zheng J, Ghazani AA, Song Q, et al. Cellular imaging and surface marker labeling of hematopoietic cells using quantum dot bioconjugates. Lab Hematol, 2006,12 (2) :94-98.

同被引文献66

  • 1吕国平,崔德健,郭英江,谭剑.介绍一种建立大鼠哮喘模型的实验方法[J].中华结核和呼吸杂志,1995,18(6):377-378. 被引量:187
  • 2王福生.3427例胃癌临床资料分析[J].胃肠病学和肝病学杂志,2006,15(1):40-41. 被引量:15
  • 3于志坚.胃癌防治研究新进展[J].交通医学,2007,21(3):225-226. 被引量:4
  • 4Chan WC, Maxwell DJ, Gao X, et al. Luminescent quantum dots for multiplexed biological detection and imaging. Curr Opin Biotechnol, 2002, 13( 1 ) :40-46.
  • 5Tholouli E, Sweeney E, Barrow E, et al. Quantum dots light up pathology. J Pathol, 2008, 216 ( 3 ) : 275-285.
  • 6Tholouli E, Hoyland JA, Di Vizio D, et al. Imaging of multiple mRNA targets using quantum dot based in situ hybridization and spectral deconvolution in clinical biopsies. Biochem Biophys Res Commun, 2006, 348(2):628-636.
  • 7Medintz IL, Uyeda HT, Goldman ER, et al. Quantum dot bioconjugates for imaging, labelling and sensing. Nat Mater, 2005, 4(6) :435-446.
  • 8Sweeney E, Ward TH, Gray N, et al. Quantitative multiplexed quantum dot immunohistochemistry. Biochem Biophys Res Commun, 2005, 374(2) :181-186.
  • 9Wu X, Liu H, Liu J, et al. Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots. Nat Biotechnol, 2003, 21 ( 1 ) :41-46.
  • 10Ness JM, Akhtar RS, Latham CB, et al. Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection. J Histochem Cytochem, 2003, 51 (8) : 981-987.

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