国产番泻叶的泻下及抗骨质疏松作用研究

Study on cathartic and anti-osteoporosis effects of native Folium sennae

目的:探讨国产番泻叶的泻下及雌性激素样抗骨质疏松作用。方法:比较国产与进口商品番泻叶提取物对小鼠排便和肠蠕动作用的影响,观察其泻下通便的效果。并通过观察对去势雌性小鼠动情周期、子宫重、股骨重的变化和血钙、血磷、降钙素含量的影响,研究由国产番泻叶制备而得的番泻总苷提取物的雌性激素样抗骨质疏松作用。结果:两种商品番泻叶提取物与生理盐水对照组相比均能显著增加墨汁推进率、缩短首次排便时间,增加6h排便量,两种商品之间无显著性差异。由国产番泻叶制备而得的番泻总苷提取物能缩短去势小鼠动情周期,有雌性激素样促进子宫增生和骨质钙化的作用。结论:国产番泻叶具有较好的泻下通便作用,并初步发现其具有雌性激素样抗骨质疏松作用。

Objective： To investigate the cathartic and anti-osteoporosis effect of native Folium sennae. Methods： Cathartic effect was assayed by measuring the action on defecation and intestinal peristalsis of mice treated with Folium sennae extracts. Inhibitory effect on osteoporosis similar to estrogen was investigated by observing estrous cycle, detecting weight of uterus and left femur, and value of plasma calcium, plasma phosphorus and calcitonin treated with purified total sennosides extracts. Results： Compared with saline control group, the group treated with Folium sennae extracts markedly enhanced peristaltic rate of intestine, shortened the time of first defecation and increased bulk of 6 hours stools. There was no significant difference between native and foreign Folium sennae on these aspects. The purified total sennosides extract shortened estrous cycle, improved weight of uterus and left femur, and decreased value of plasma calcium in ovariectomized mice. Conclusion： The results indicate that native Folium sennae possesses obvious cathartic effect, and the purified total sennosides extract from native Folium sennae exhibits inhibitory effect on osteoporosis similar to estrogen.