摘要
目的探讨脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)及其受体酪氨酸激酶受体B(tyrosine kinase B,TrkB)在变应性鼻炎发病中的作用。方法采用6～8周健康SD大鼠,随机分成实验组20只和对照组10只,以卵清蛋白致敏激发制成变应性鼻炎模型,免疫组化染色检测鼻黏膜中BDNF及TrkB的表达及分布。结果BDNF及TrkB在实验组和对照组大鼠鼻黏膜中主要表达在鼻黏膜上皮细胞、腺细胞及导管细胞和固有层炎性细胞的胞质内及部分腺细胞胞核内;实验组和对照组鼻黏膜中BDNF的积分光密度(integrated optical density,iOD)值分别为77344.17±8567.02和54124.82±18515(P<0.05),TrkB的iOD值分别为93087.57±17001.97和74914.78±15895.92(P<0.05),均具有显著性差异。BDNF与TrkB呈正相关(rs=0.589,P<0.01)。结论BDNF与变应性鼻炎的发生有着密切的联系,可能主要通过与TrkB结合而参与变应性鼻炎的发生、发展。
OBJECTIVE To investigate the role of brain-derived neurotrophic factor（BDNF） and its receptor TrkB in the development of allergic rhinitis（AR）. METHODS SD rats were randomly divided into allergic rhinitis group（AR group, n = 20） and control group （Con group, n = 10）. A model of allergic rhinitis in health SD rat was established by using ovalbumin intraperitoneal immunization and nasal antigen challenge. The nasal mucosa obtained from 18 out of 20 AR models as well as 10 normal controls were studied with immunohistochemistry （Envision System） method to observe the expression and distribution of BDNF and TrkB. RESULTS Immunohistochemical staining showed that the distribution of BDNF and TrkB in nasal mucosa from normal rats was in accordance with that in the nasal mucosa from rats with allergic rhinitis on the whole. The study showed that immunoreactivity to BDNF （or to TrkB） was present both on the surface and in the cytoplasm of epithelial cells, glandular cells and their duct cells. The statistical analysis of imumunohistochemical staining showed that the IOD of BDNF in rat nasal mucosa with allergic rhinitis （77344.17±8567.02） was obviously higher than that in the normal rat nasal mucosa （54124.82± 18515）. There was a statistical significance between the two groups （t= 3.053, P〈 0.05）; The IOD of TrkB in nasal mucosa with allergic rhinitis （93087.57 ± 17001.97） was higher than that in the normal rat nasal mucosa （74914.78 ± 15895.92）.There was also a statistical significance between the two groups （t = 2.771, P〈 0.05）. BDNF was significantly positively correlated with TrkB （rs = 0.589, P〈 0.01）. CONCLUSION Brain-derived neurotrophic factor may play an important role in the development of allergic rhinitis by binding with TrkB.
出处
《中国耳鼻咽喉头颈外科》
北大核心
2008年第5期259-261,共3页
Chinese Archives of Otolaryngology-Head and Neck Surgery
