进境粮谷中截获的检疫性杂草种子生活力测定

VIABILITY TEST OF SOME QUARANTINE WEED SEEDS INTERCEPTED FROM IMPORT GRAINS

[目的]测定进境粮谷中截获的检疫性杂草种子的生活力,为研究进境检疫性杂草种子的灭活方法提供基础理论数据。[方法]采用四唑染色测定方法(TTC)测定。[结果]测定出进境粮谷中截获的7种检疫性杂草种子的生活力。其中:豚草(Ambrosia artemisifolia L.):72.0%,三裂叶豚草(Ambrosia trifida L.):73.2%,法国野燕麦(Avena ludoviciana Dur.):22.0%,刺蒺藜草(Cenchrus echinatus L.):89.0%,锯齿大戟(Euphorbia dentata Michx.):30.5%,假高粱(Sorghum halepense Pers.):美国产100.0%,阿根廷产81.0%,苍耳属(Xanthium spp.):53.0%。[结论]采用种子四唑染色方法测定杂草种子的生活力,不受种子休眠限制,不需摸索其萌发所需最适宜的温湿度条件,方法简便快捷,结果准确。

The viabilities of quarantine weed seeds intercepted from import grains were tested by 2,3,5-Triphenyl Tetrazolium Chloride （TTC） method for the study of killing method. The viabilities of the seeds are as follows： 72.0% for A mbrosia artemis ifolia L., 73.2% for Ambrosia trifida L., 22.0% for Arena ludoviciana Dur., 89.0% for Cenchrus echinatus L, 30.5% for Euphorbia dentata Michx., 100.0% for Sorghum halepense Pers. from USA and 81.0% from Argintina, and 53.0% for Xanthium Spp.. It showed that TTC method has several advantages such as simple, accurate and non-considering of seed dormancy, temperature and humiditv of seed germination.