腹腔镜结直肠癌手术外周血细胞角蛋白20mRNA表达的研究

Analysis of CK20mRNA in patient underwent laparoscopic surgery for colorectal cancer

目的研究细胞角蛋白20mRNA（CK20mRNA）在腹腔镜结直肠癌手术患者外周血中的表达情况，探讨腹腔镜治疗结直肠癌手术对肿瘤微转移的影响。方法将44例结直肠癌患者采用单盲随机方法分为腹腔镜组25例与开腹组19例，采用逆转录聚合酶链反应检测患者术前、术中、术后外周血中CK20mRNA的表达，对132例次标本进行CK20mRNA检测的研究。结果腹腔镜组CK20mRNA阳性表达25例次，阴性表达50例次；开腹组：CK20mRNA阳性表达22例次，阴性表达35例次；2组间比较无统计学意义。结论腹腔镜结直肠癌手术患者外周血术前、术后CK20mRNA表达与开腹手术无统计学意义。腹腔镜结直肠癌手术在肿瘤微转移方面是安全、可行、有效的。

Objective To study the possible influence of laparoscopic surgery on tumor micrometastasis via detecting the expression of CK20mRNA in patients with colorectal cancer. Methods Forty-four cases of colorectal cancer were divided into two groups： the laparoscopic surgery group（25 cases） and open surgery group（ 19 cases）. Blood samples were obtained 24 hours before the operation, intra-operation after finishing anastomosis, and 48 hours after operation respectively. RT-PCR was applied to detect the expression of CK20mRNA before-, intra- and post- operation. Results In the laparoscopic surgery group, 25 cases were CK20mRNA positive, whereas 50 cases were negative. The before-, intra-, post-operation expression of CK20mRNA positive cases were 8, 9, 8, respectively. In the open surgery group, 22 cases were CK20mRNA positive, whereas 35 cases were negative. The before-, intra-, post-operation expression of CK20mRNA positive cases were 9, 6, 7, respectively. Conclusion There was no significant difference of the expression of CK20mRNA before- and post-operation between the two groups. Laparoscopic surgery for colorectal cancer had little influence on tumor micremetastasis.