海湾扇贝酶解产物清除自由基活性的研究

Study on Free Radical Scavenging Activity of Enzymatic Hydrolysate of Ar-gopecten irradians Edible Part

利用双酶(胰酶0.3%和枯草杆菌中性蛋白酶0.8%)。在50℃、pH值7.0～8.0、料水比1:3的条件下,对海湾扇贝肉酶解4h后制备得到产物.该酶解产物对羟自由基的清除活性IC_(50)为2.01 mg/mL,对超氧阴离子的清除活性IC_(50)为9.54 mg/mL,对DPPH自由基的清除活性IC_(50)为5.90 mg/mL。Sephadex G-15(1.6 cm×68 cm)凝胶色谱结果表明,该酶解产物中分子质量分布在1450～288 u,肽链长度在13.2～2.6的组分具有较强的清除羟自由基和DPPH自由基活性。

Hydrolysate with free radical scavenging activity was prepared from the edible part of Argopecten irradians by pancreatin （0.3%） and neutral protease （0.8%）, under the condition of 50℃ ,pH7.08 -8.0,solid water ratio 1 ： 3 and reaction time 4h. The hydrolysate showed an IC50 of 2.01mg/mL for hydroxyl radical, an IC50 of 9.54mg/mL for superoxide radical and an IC50 of 5. 90mg/mLfor DPPH radical. The hydrolysate was fractionated with a Sephadex G--15 column （1.6cm×68cm）. The absorbance of each fractionated tube was measured at 280nm and its molecular weight distribution was calculated according to a regressive equation between the elution volume and the logarithm of the molecular weight. Gel filtration of the hydrolysate on Sephadex G-15 chromatogram yielded five absorbance peaks. Peak C exerted the strongest hydroxyl radical and superoxide radical scavenging activity, the molecular weight ranged from 1 450 u to 288 u, and the lengths of peptide linkage located betweenl3.2-2.6.