骨髓间充质干细胞对豚鼠皮肤光老化治疗作用的研究

Research of bone marrow mesenchymal stem cells curing photoaging of guinea pig skin

目的:探讨骨髓间充质干细胞(Bone Marrow Mesenchymal Stem Cells,BMSCs)皮肤光老化的修复作用,为皮肤光老化的治疗提供新的治疗途径。方法:UVA,UVB紫外线灯同时照射豚鼠,每天2h,连续照射50天,制备皮肤光老化模型;紫外线照射2周后,在豚鼠皮肤照射区,局部多点皮内注射DAPI标记的第3代豚鼠BMSCs 0.8ml(1×105个/ml),每周一次。注射后24h、5天及10天观察豚鼠皮肤变化,同时取照射区的皮肤制备冰冻切片,激光共聚焦显微镜下观察DAPI标记的BMSCs在紫外线照射区皮肤组织内的迁徙及定居情况。结果:与对照组相比,随照射时间的延长,未治疗组皮肤光老化症状逐渐加深,出现皱纹,皮肤松弛、修复性增厚,皮屑,色素沉积,皮革样外观;BMSCs治疗组在治疗前出现的症状与未治疗组相同,治疗后上述症状逐渐消失,未出现色素沉着。激光共聚焦显微镜下观察,注射后24h,BMSCs出现在真皮层;注射后5天BMSCs出现在真皮层以及毛囊等组织;注射后10天广泛弥散在皮肤各层,包括角质层。结论:BMSCs对皮肤光老化有明显的延缓及修复作用。

Objective To research bone marrow mesenchymal stem cells（BMSCs） curing photoaging of guinea pig skin to provide a new way of curing skin photoaging. Methods Irradiated guinea pigs 2 hours per day for 50 days using UVA and UVB ultraviolet radiator to establish skin photoaging model.After 2 week radiation executed multiple intradermal injections of the third-generation BMSCs（0.8ml,1×10^5/ml） marked by DAPI into the ultraviolet radiation area of guinea pig skin every week.Observed the skin 24h,5d and 10d after injection,and maked frozen section from the ultraviolet radiation area of guinea pig skin.Observed migration and ecesis of guinea pig skin by laser scanning confocal microscope the BMSCs marked by DAPI in ultraviolet radiation area of Results Compared with control group,skin photoaging symptoms of the no-treatment group increased with irraditive time,wrinkles, cutis laxa,scurf reparative thickening, pigment deposition and leather appearance appeard.The same symptom of the treatment group gradually dissappered and no pigment deposition appeard in the treatment group.Using laser scanning confocal microscope the BMSCs marked by DAPI were observed appearing in dermis 24h after the injection,appearing in dermis,hair follicle,etc,5d after the injection,becoming dispersedly distributed in the entire layers of the skin including stratum corneum 10d after the injection. Conclusion BMSCs could obviously delay and cure skin photoaging.