己烯雌酚对体外培养正常人表皮黑素细胞的作用

Effects of Diaethylstilbestro(DES) on cultured human melanocytes in vitro

目的:观察不同浓度外源性雌激素己烯雌酚(Diaethylstilbestro,DES)对体外培养人正常黑素细胞的细胞增殖及酪氨酸酶活性的影响。方法:采用MTT法测定黑素细胞增殖情况,参考NakajimaM方法测定酪氨酸酶活性。结果:不同浓度己烯雌酚对体外培养人黑素细胞增殖及酪氨酸酶活性均有促进作用。结论:己烯雌酚能促进人黑素细胞增殖及酪氨酸酶活性,对相关皮肤色素性疾病可能有一定的治疗作用。

Objective Establishing the culture of human normal melanocytes In vitro,and studying the effects of Diaethylstilbestro （DES） on cultured human melanocytes. Methods Viability of melanocytes were measured by MTT method;tyrosinase activity was measured by utilization of L-Dopa as the substrate （Nakajima M,method ）. Results Different dosage of Diaethylstilbestro （DES）induced a strong promotion on melanocytic viability and tyrosinase activity. Conclusion Diaethylstllbestro （DES）can promote melanocytic proliferation and tyrosinase activity. Diaethylstilbestro might have some therapentical effects on correlated pigmented skin diseases.