40日龄昆明小鼠主要生物学特性标准化研究

Standardization of Biological Properties of KM Mice aged 40 days

目的:为建立昆明小鼠生物学特性标准化资料提供实验依据。方法:在昆明小鼠封闭群中随机抽取40日龄昆明小鼠102只,测定其生长发育指标,采用生化分析仪测定血常规数据、血液生化值,制作血膜片进行白细胞计数和分类。结果:40日龄昆明小鼠平均体重为(22.080±3.032)g,平均体全长为(182.35±7.043)mm、头长(25.59±2.600)mm、尾长(91.29±4.404)mm,WBC计数(7.953±3.335)×109/L,WBC分类NE28.32%±12.13%、LY68.31%±12.75%、MO3.37%±3.01%,血常规、生化值结果稳定可靠。结论:确定了江中40日龄昆明小鼠主要生物学特性,为中国昆明小鼠生物学特性标准化提供可靠数据。

Objective： To afford experimental basis to establishing the datum of the standardization of biological properties of KM Mice. Methods： 102 litters of 40days aged KM mice were randomly selected to detect the indexes of growth. The datum of blood routine examination and blood biochemical indexes were detected by biochemical analyzer. Leucocyte was taken count of and sorted by making blood patch. Results： the average weights of KM mice aged 40 days was （22. 080 ± 3. 032）g, the average body length was （ 182. 35 ± 7. 043）mm, the head length was （25.59 ± 2. 600）mm, the tail length was （91.29 ± 4.404 ）mm and the WBC counting average was 7. 953 ± 3. 335 × 10^9/L.The category average NE was 28.32 % ± 12.13 %, the LY was 68.31% ± 12.75 %, the MO was 3.37 % ± 3.01%, and the blood routine and biochemical value results were stable and reliable. Conclusion：the biological properties of KM mice were studied. It provided a reliable evidence for the standardization of KM mice in China.