吸入肝素对哮喘患者诱导痰液中ECP、TNF-α、sIL-2R水平的影响

Effect of heparin inhalation on ECP,TNF-α,sIL-2R levels in asthmatic patients' induced sputum

目的:诱导测定哮喘患者痰液ECP、TNF-α、sIL-2R水平,评价吸入肝素防治哮喘的作用。方法:采用酶联免疫法及荧光免疫法对40例轻中度哮喘患者在吸入肝素前后测定痰液中嗜酸细胞阳离子蛋白(ECP)、肿瘤坏死因子α(TNF-α)、可溶性白细胞介素2受体(sIL-2R)水平。结果:吸入肝素后诱导痰液ECP水平由(298±150)μg/L降至(147±95)μg/L、TNF-α由(287±147)ng/L降至(139±59)ng/L、sIL-2R由(498±127)kU/L降至(358±107)kU/L。哮喘患者吸入肝素前ECP与TNF-α,sIL-2R均呈显著正相关(r=0.58,0.62,P<0.05)。吸入肝素后各指标间无明显相关性。结论:吸入肝素后哮喘患者诱导痰液中ECP、TNF-α、sIL-2R浓度比吸入肝素前明显降低。吸入肝素可明显抑制气道炎性反应,其作用理机可能与抗炎作用有关。

Objeαive：To investigate the effeα of inhaled heparin on eosinophil cationic protein （ECP）, tumor necrosis factor α （ TNF - or） and soluble interleukin 2 receptor （ sIL - 2R） levels in asthmatic patients＇ induced sputum. Methods：The ECP, TNF- α and sIL -2R levels in induced sputum in 40 patients with mild- moderate asthma were deteαed before and after heparin inhalation by ELISA and fluorescence immunity methods. Results： The levels of ECP, TNF-α and sIL -2R were （298 ± 150）μg/L, （287± 147） ng/L and （498± 127） kU/L before heparin inhalation, and （ 147±95 ） μg/L, （ 139 ± 59 ） ng/L and （ 358 ± 107 ） kU/L after heparin inhalation. Before heparin inhalation, there were significantly positive correlations between the ECP level and the TNF - α levels （ r =0.58, P 〈0.05 ） and between the ECP level and the sIL -2R levels （ r =0.62, P 〈0.05） . No relation was found among them after heparin inhalation. Conclusion ： The decrease of the ECP, TNF - α and slL -2R levels in sputum after heparin inhalation can inhibit the inflammatory reaαion of respiratory passage in asthmatic patients, indicating that heparin may be of anti - inflammatory effeα in asthmatic patients.