当归注射液有效成分的抗低氧保护作用

ANTI-HYPOXIA PROTECTIVE ROLE OF THE EFFECTIVE COMPONENT EXTRACTED FROM ANGELIA INJECTION

目的:研究梯度分离的当归注射液有效成分(5-HMF)对小鼠及体外培养的ECV304细胞的抗低氧保护作用。方法:观察常压低氧和低压低氧下小鼠存活时间;通过MTT比色法检测ECV304细胞经低氧后的细胞存活率、并以台盼蓝染色检测细胞死亡率来评价药效。结果:与低氧组比较,加药低氧后,小鼠存活时间延长(P<0.05);低氧后细胞存活率降低,死亡率升高,而加入当归注射液有效成分(5-HMF)处理的细胞存活率明显高于低氧对照组(P<0.01),死亡率亦明显降低(P<0.01)。结论:当归注射液有效成分(5-HMF)对小鼠及体外培养的ECV304细胞均具有抗低氧保护作用。

Aim： To investigate anti-hypoxia protective roles of the effective component extracted from angelia injection using hypoxia injury model in mice and ECV304 cells separately. Methods： The survival time of mice was observed separately under normobaric and hypobaric hypoxia. The activity of ECV304 cells was tested by MTT assay, and the mortality rate was examined by Trypan blue exclusion assay to evaluate the pharmacodynamic effects. Results： After exposed to hypoxia the survival time of mice was increased in medicine groups, compared with the control groups（ P 〈 0.05）. The cell survival rate was decreased and the cell mortality rate was increased after cells were exposed to hypoxia, while the cell survival rate was significantly increased（ P 〈 0.01 ）, and the cell mortality rate was significantly decreased（P 〈 0.01 ）in the medicine groups compared with the control groups. Conclusion： The effective component extracted from angelia injection can protect against the injury induced by hypoxia.