桑白皮提取物对糖尿病大鼠坐骨神经结构影响的实验研究

EXPERIMENTAL STUDY ON THE PREVENTIVE AND TREATMENT FUNCTION OF CORTEX MORI FOR PERIPHERAL NERVOUS LESION AT THE EARLY STAGE OF DIABETES RATS

目的:观察桑白皮提取物对糖尿病(DM)大鼠周围神经早期病变的防治作用。方法:采用四氧嘧啶诱发糖尿病大鼠模型,成模后依据血糖值将大鼠随机分为模型组、桑白皮提取物(大、小剂量)组,弥可保对照组,另设空白对照组,分别灌胃,每天一次,2月后对照观察各组大鼠血糖(FBG)、体重(W)、对坐骨神经进行病理学检测,观察髓鞘面积、髓外纤维、髓鞘横断面面积、透射电镜观察坐骨神经超微形态的变化。结果:桑白皮提取物能调节血糖、有效地增加糖尿病性大鼠坐骨神经髓鞘面积、髓外纤维、髓鞘横断面面积,减轻神经髓鞘水肿,减轻坐骨神经的病变。结论:桑白皮提取物可明显缓解DM大鼠周围神经的早期病变,有一定的神经修复作用,综合疗效优于弥可保。

Aim： To observe the preventive function of cortex mori for peripheral nervous lesion at the early stage in diabetes rats, and probe into the mechanism of that formula. Methods： Set up the diabetes rat model evoked by alloxan. According to different blood sugar values, randomly divide the rats into normal group, model group, cortex mori（high dosage and low dosage） group and methylcohala-min control group, respectively administer the rats with saline and cortex mori of different dosages by ig（ 1.875 g/kg, 0.625 g/kg）, while 300 μg/kg methylcobalamin for control group, one time each day. Two months after the administration, determine the FBG, body weight, SOD and MDA in blood serum of rats in each group, observe the changes on final product of glycosylation, CGMP and CAMP of sciatic nerve and the synapsin of rats＇ sciatic nerves. Conduct the pathological observation on area of myelin sheath, extramedullary fiber and the cross section of myelin sheath of sciatic nerves. And observe the changes of ultrafine form of sciatic nerve through transmission electron microscope. In the mean time, determine the MNCV, SNVC and SL, and the tail-flicking test should be undertaken for checking the sensory nerve. Results： Cortex mori can effectively enlarge the area of myelin sheath, extramedullary fiber and the cross section of myelin sheath.Conclusion： Cortex mori can obviously ease up the pathological changes of peripheral nerve at the early stage of the diabetes rats, and the overall curative effect is better than that of methylcobalamin.