酶法拆分N-乙酰-D,L-丙氨酸反应控制步骤

RATE - CONTROLLING STEP OF OPTICAL RESOLUTION ON N - ACETYL-D, L-ALANINE WITH POROUS IMMOBILIZED ASPERGILLUS ORYZAE CELLS

研究了固定化米曲霉菌光学拆分N-乙酰-D,L-丙氨酸反应过程的速率控制步骤。在近似无限浴的条件下,通过测量间歇拆分反应产生的L-丙氨酸的浓度变化规律,判断反应过程中的速率控制步骤。结果表明,底物浓度和反应温度是影响速率控制步骤的重要因素,用得到的有效扩散系数进行模型计算,结果与实验值基本一致。

The rate-controlling step of resoluting N-acetyl-D, L-alanine with porous immobilized Aspergillus Oryzae cells was studied. In the batch reaction with high ratio of substrate to catalysts, the rate ?controlling step was determined by measuring the concentration variation of produced L-alanine in solution. The results showed that the substrate concentration and temperature were important factors which influenced the rate - controlling step. The results of model calculated with effective diffusion coefficient obtained were in agreement with experiment data.