树突状细胞激活的CTLs在裸鼠体内外诱导抗喉癌作用

Anti-laryngocarcinoma Effect of Dendritic Cells-activated CTLs in Vivo and in Nude Mice

目的探讨树突状细胞(Dendritic cells,DCs)激活的细胞毒性T细胞(eytotoxic T lymphocytes,CTLs)在裸鼠体内外能否诱导抗喉癌免疫。方法用细胞因子从健康人外周血诱导DCs,MTT法检测DCs激活的CTLs在体外对喉癌细胞的作用。将裸鼠接种CTLs,1w后用喉癌细胞攻击,观察成瘤潜伏期、成瘤率、瘤重量及瘤体积。结果用细胞因子能从健康人外周血诱导大量DCs,该DCs激活的CTLs在体外对喉癌细胞产生了高效而特异的抑制及杀伤作用(P<0.01)。在裸鼠体内,负载抗原的DCs激活的CTLs有免疫保护作用,其成瘤率减低(84%,P<0.01),潜伏期延长(14±2d,P<0.01),瘤重及瘤体积均显著降低(0.47±0.10g,12.64±3.98mm^3,P<0.01)。结论DCs激活的CTLs能在裸鼠体内外诱导高效的抗喉癌作用。

Objective To investigate the effect of eytotoxic T lymphoeytes （CTLs） activated by dendritic cells （DCs） on inducing antitumor response in vivo and in nude mice. Methods DCs were induced by cytokines from the peripheral blood of healthy people. The antitumor effect on laryngocarcinoma of CTLs was observed in vivo by MTT method, and the stage of latency, tumor generative rate, weight and volume of tumor were determined in nude mice with laryngocarcinoma cells. Results DCs can be induced from the peripheral blood of healthy people into the CTLs, which can induce the effective antitumor response on laryngocarcinoma in vivo. Additionally, CTLs have the protective effect for the nude mice, with the decreased tumor generattive rate （84%, P 〈 0.01）, prolonged the latency stage （14 ± 2d, P 〈 0.01） and decreased weight and volume of tumor significantly compared with control（0.47 ±0.10g,12.64±3.98mm^3, P〈 0.01）. Conclusions DCs-indueed CTLs have the effective immunoresponse on laryngocareinoma in vivo and in nude mice.