摘要
从分离群体的选择、DNA分子标记、基因文库的构建、目标基因的分离、鉴定等方面综述了基于DNA分子标记的植物功能基因的分离方法。
The methods of separating functional gene of plants based on DNA molecular marker were summarized, including the choice of segregation population, DNA molecular marker, construction of gene library, the separation and identification of target gene.
出处
《江西农业学报》
CAS
2008年第5期17-20,24,共5页
Acta Agriculturae Jiangxi
关键词
图位克隆
分子标记
多态性
CDNA文库
基因组文库
文库筛选
Map -based cloning
Molecular marker
Polymorphism
cDNA library
Genomic library
Library screening