一种新的Lowry氏蛋白质定量测定方法

A new Lowry's technique for quantitative measurement of protein

液体闪烁计数中的淬灭效应被应用于Lowry氏蛋白质定量测定。结果表明:不同浓度的显色蛋白样品与仪器计数效率之间存在较好的线性关系。实验证明液闪测定法较传统的分光光度计测定法具有误差小、可测范围大、便于自动化分析等特点。

According to the quenching principle in beta ray measurement, liquid scintillation eonnters were used for quantitative measurement of protein. The results show linear relationship between the colored protein samples with different concentrations and the counting rate of LSC. It has been proved that LSC method is less erroneous and has larger measurement range than the traditional photoelectric colorimetry, and the analysis is easy to be automatized.