二氧化硅粉尘对人红细胞谷胱甘肽和乳酸脱氢酶的影响

EFFECT OF SILICA DUST ON GLUTATHIONE AND LACTIC DEHYDROGENASE CONTENTS OF HUMAN ERYTHROCYTE

本文旨在探讨二氧化硅粉尘对人血液红细胞谷胱甘肽（GSH）含量和乳酸脱氢酶（LDH）活力的影响及其抗氧化损伤作用。随机抽取27名身体健康，无粉尘接触史人员组成对照组，某石英砂厂接尘工人43人组成接触组和36名矽肺病人作为病人组。采用改良DTNB显色法测定红细胞GSH含量和L→P反应固定时间法，测定红细胞LDH活力。结果显示：二氧化硅粉尘接触者和矽肺病人红细胞GSH含量和LDH活力显著高于对照组（P＜0．01）。接矽尘工龄与红细胞GSH含量和LDH活力之间呈正相关（r=0．3965，r=0．4133，P＜0.05）。表明二氧化硅粉尘可致机体氧代谢产物负荷蓄积。血液抗氧化剂载体-红细胞功能活跃，GSH含量和LDH活力升高，有助于机体对二氧化硅粉尘所致氧化性损伤的代偿和适应。

In order to observe the effects of silica dust on glutathione (GSH) and lactic dehydrogenase(LDH) of human erythrocytes and the role of these parameters in antioxygen injury,27 randomlysampled healthy subjects were taken as the controls, 43 dust exposed workers of a quartz sandplant were taken as the expcsed group,and 36 silicotics as the patient group, Using the improvedDTNB method and L-P fixed reactive time,the contents of GHS and LHD of blood erythrocyteswere measured. the results showed that GSH and LDH contents of the exposed group and the patient group were obviously higher than those of the controls (P＜0.01). By the corre1ation analysisbetween years of exposure and the contents of GSH and LDH in erythrocytes,positive correlationswere found (r= 0. 3965,r =0. 4133 P<0. 05 ). The results indicated that silica dust could induce theaccumulation of the load of the oxygen metabolites. The carrier of antioxygen metabolites in blood,which may activate the function of erytkrocytes and elevate the GSH content and LDH activity,might compensate the antioxygen injury induced by silica dust.