喉癌人乳头瘤病毒感染和p53蛋白表达的研究

Study on HPV infection and p53 protein expression in laryngeal carcinoma

对44例喉鳞癌和16例声带息肉患者采用原位核酸杂交技术探测其人乳头瘤病毒（HPV）6B、11、16、18型DNA同源序列及LSAB免疫组化法探测其p53蛋白的表达。结果：①喉癌与HPV16／18杂交阳性率为43．2％（19／44），声带息肉为12．5％（2／16）（P＜0．05）；②喉癌p53蛋白阳性率为56．8％（25／44），声带息肉全部为阴性；③喉癌HPV16／18杂交及p53蛋白均呈阳性反应者12／44，占HPV16／18杂交阳性标本的63．2％（12／19），占p53蛋白阳性反应标本的48．0％（12／25）。表明：HPV16／18型感染和p53蛋白表达与喉癌的发生有密切关系，并且二者可能有协同作用。

In order to evaluate the role of human papillomavirus (HPV) infection and p53protein expression in laryngeal squamous cell carcinoma (LSCC), the insituhybridization and thelabelled streptavidin biotin (LSAB) immunohistochemical method were used to detect the presenceof HPV genomes 6B, 11, 16, 18 and expression of p53 protein respectively in 44 specimens ofLSCC. And 16 specimens of laryngeal polyp were selected as controls. Results: 1. HPV genomes16/18 were detected in 19 (43. 2％ ) of 44 LSCCs and in 2 (12. 5％) of 16 laryngeal polyps (P<0. 05). 2. 25 (56. 9％) of 44 specimens of LSCC were p53 protein positive whereas the laryngealpolyps were all p53 negative. 3. 12 (63.2％) of 19 HPV 16/18 DNA-positive specimens of LSCCshowed p53 protein expression and 12 (48% ) of 25 p53 protein positive specimens of LSCC showedHPV 16/18 infection. Conclusion: Expression of p53 protein and infection of HPV l6, 18 may playa role in carcinogenesis of LSCC, respectively or simultaneously.