摘要
目的对中国HIV感染长期不进展者(LTNP)CD4^+CD25^+Foxp3^+调节性T细胞水平及其与疾病进展相关性进行研究,探讨CD4^+CD25^+Foxp3^+调节性T细胞在LTNP保护机制中发挥的作用。方法选取74名HIV-1感染者(LTNP、典型进展HIV组、AIDS组)及16名健康对照,应用流式细胞仪胞内染色技术在单细胞水平检测CD4^+CD25^+Foxp3^+调节性T细胞表达水平,分析其与CD4^+T细胞数量、病毒载量、淋巴细胞活化、凋亡水平的相关性。结果中国HIV感染LTNP CD4^+CD25^+Foxp3^+T细胞百分率明显低于典型进展HIV、AIDS组及健康对照组(P〈0.05)。HIV/AIDS患者CD4^+CD25^+Foxp3^+T细胞百分率与CD4^+T细胞显著负相关(r=-0.509,P〈0.001),与病毒载量明显正相关(r=0.414,P〈0.01),与CD4、CD8^+T细胞表面CD38、CB95表达水平明显正相关(P〈0.05),与CD4、CD8^+T细胞表面HIA.DR表达无显著相关性。结论中国HIV感染LTNP CD4^+CD25^+Foxp3^+调节性T细胞百分率明显低于典型进展者,提示调节性T细胞与LTNP保护机制相关。
Objective To study the association of CD4^+CD25^+Foxp3^+ regulatory T cells with the HIV long term non-progressors(LTNP) in China. Methods Seventy-four HIV-1 infected patients ( LTNP group, HIV group and AIDS group)and 16 normal controls were enrolled and the frequency of CD4^+CD25^+Foxp3^+ regulatory T cells were detected by flow cytometry. To study the correlation between CD4^+CD25^+Foxp3^+ regulatory T cells and disease progression, the absolute CD4^+ T cells, viral load, apoptosis and activation of T cells were also examined. Results The frequency of CD4^+CD25^+Foxp3^+ regulatory T cells in LTNP group was significantly lower than that in HIV and AIDS group ( P 〈 0.05 ). The frequency of CD4^+CD25^+Foxp3^+ regulatory T cells was inversely related to CD4^+ T cells and closely related to viral load and CD38, CD95 expression on CD4, CD8^+T cells ( P 〈 0.05 ). Conclusion The frequency of CD4^+CD25^+Foxp3^+ regulatory T cells of HIV infected LTNP is significantly lower than typical progressors, which indicates that alternation of regulatory T cells may play a protective role in LTNP.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2008年第5期450-453,共4页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金(30600532)
国家重点基础研究发展(973)计划(2006CB504206)
重大基础研究前期研究专项(2004CCA02000)