非霍奇金淋巴瘤中EB病毒BNLF_1片段及其表达产物的检测及意义

THE SIGNIFICANCE OF DETECTING EPSTEIN BARR VIRUS BNLF 1 FRAGMENT AND ITS EXPRESSION IN NON HODGKIN'S LYMPHOMA

为研究ＥＢ病毒与非霍奇金淋巴瘤（ＮＨＬ）的关系，采用ＰＣＲ、原位杂交、ＬＳＡＢ（ｌａｂｅｌｉｎｇｓｔｒｅｐｔａｖｉｄｉｖｉｎｂｉｏｔｉｎ）免疫组化技术，检测３２例ＮＨＬ和３３例反应性增生淋巴结中的ＥＢＶＢＮＬＦ１基因片段及其表达产物潜伏膜蛋白（ＬＭＰ１）。ＮＨＬ中ＥＢＶＢＮＬＦ１ＰＣＲ扩增阳性率为５３％，明显高于反应性增生淋巴结组２７％（Ｐ＜０．０５），其中５例Ｔ细胞淋巴瘤全部阳性。１７例ＰＣＲ阳性的ＮＨＬ中５例原位杂交阳性。ＬＭＰ１检测仅２例阳性。结果表明本组病例半数以上ＮＨＬ中有ＥＢＶ感染，可能与肿瘤的发生发展有一定关系。

In order to study the association between Epstein Barr virus(EBV) and non Hodgkin's Lymphoma(NHL), PCR, in situ hybridization(ISH) and LSAB inmmunohistochemical technique were applied to detect EBV BNLF 1 fragement and its expression product latent membrane protein(LMP 1) in 32 cases of NHL and 33 cases of reactive hyperplastic lymph nodes(RHLN). Results showed that the detection rate of BNLF 1 fragment by PCR was 53% in 32 cases of NHL, significantly higher than that of RHLN group 27%(P<0.05). 5 cases of T cell lymphoma were all PCR positive. 5 of the 17 PCR positive cases were ISH positive. LMP 1, expression product of BNLF 1 gene, was demonstrated only in two cases of this group of NHL. Our results suggested that EBV infection existed in more than one half cases of this group of NHL and might be significant in the pathogenesis and development of lymphoma.