日本血吸虫成虫抗原基因的克隆和序列分析

CLONING AND SEQUENCING OF ANTIGENIC GENE OF ADULT WORM OF SCHISTOSOMA JAPONICUM (CHINESE STRAIN)

本实验在用感染兔血清和单克隆抗体对日本血吸虫（大陆株）成虫ｃＤＮＡ表达文库进行筛选的基础上。

Using infective rabbit sera (IRS) and two strains of monoclonal antibodies as catching antibody probes,the authors have immunologically screened cDNA expression library of Schistosoma japonicum (adult worm). Eight positive clones were obtained. The gene inserted of a positive clone was subcloned and its sequence was also analyzed.