摘要
应用流式细胞术(FCM)检测36例单纯疱疹性角膜炎(HSK)患者外周血30项免疫表型的变化,并与8例正常人进行对照。结果显示HSK基质型与溃疡型CD8、HLADP、HLADR抗原表达阳性细胞明显高于正常人,基质型CD10也高于正常人。HSK基质型与溃疡型CD9、CD45RA抗原表达阳性细胞明显低于正常人,溃疡型HLAABC也低于正常人,其他抗原表达阳性细胞与正常人无差异;HSK基质型CD10、CD69、CD71抗原表达阳性细胞显著高于HSK溃疡型组,其他抗原表达阳性细胞两组无差异。此结果提示机体免疫功能紊乱及自身免疫反应可能是决定HSK是否发生及发生后HSK表现的临床类型的重要因素。HSK基质型机体可能具有强的激活免疫细胞的能力。
The 30 immune epitopes expressing in patients with herpetic keratits were investigated by flow cytometry comparing with the normal people. The results showed that the expression rate of CD8,HLA DP,HLA DR in patients with both stromatic and ulcer herpetic keratitis,also CD10 in stromatic herpetic keratitis, were higher than in normal people. And the expression rate of CD9, CD45RA in patients with both stromatic and ulcer herpetic keratitis, also HLA ABC in ulcer herpetic keratitis,were lower than in normal people. The other epitopes in patients with both stromatic and ulcer herpetic keratitis had no difference with normal people. The expression rate of CD10,CD59,CD71 in patients with stromatic herpetic keratitis were higher than those in patients with ulcer herpetic keratitis. There were no difference in the other epitopes between the stromatic and the ulcer herpetic keratitis group. These results suggest that the immune disorder and autoimmunity may play an important role in the occurrence and clinical types of herpetic keratitis. The strong activating capacity for immunocyte were showed in individual with parenchymatous herpetic keratitis and the insufficient activating capacity for immunocyte were showed in individual with ulcer herpetic keratitis.
出处
《中山医科大学学报》
CAS
CSCD
1997年第4期275-278,共4页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
广东省自然科学基金
关键词
角膜炎
疱疹性
免疫表型
分型
keratitis,herpetic/immunology
immunophenotyping