单疱病毒性角膜炎患者外周血免疫表型的差异

THE CHANGE OF IMMUNE EPITOPE FROM THE PERIPHERIAL BLOOD OF PATIENTS WITH HERPES SIMPLEX KERATITIS

应用流式细胞术（ＦＣＭ）检测３６例单纯疱疹性角膜炎（ＨＳＫ）患者外周血３０项免疫表型的变化，并与８例正常人进行对照。结果显示ＨＳＫ基质型与溃疡型ＣＤ８、ＨＬＡＤＰ、ＨＬＡＤＲ抗原表达阳性细胞明显高于正常人，基质型ＣＤ１０也高于正常人。ＨＳＫ基质型与溃疡型ＣＤ９、ＣＤ４５ＲＡ抗原表达阳性细胞明显低于正常人，溃疡型ＨＬＡＡＢＣ也低于正常人，其他抗原表达阳性细胞与正常人无差异；ＨＳＫ基质型ＣＤ１０、ＣＤ６９、ＣＤ７１抗原表达阳性细胞显著高于ＨＳＫ溃疡型组，其他抗原表达阳性细胞两组无差异。此结果提示机体免疫功能紊乱及自身免疫反应可能是决定ＨＳＫ是否发生及发生后ＨＳＫ表现的临床类型的重要因素。ＨＳＫ基质型机体可能具有强的激活免疫细胞的能力。

The 30 immune epitopes expressing in patients with herpetic keratits were investigated by flow cytometry comparing with the normal people. The results showed that the expression rate of CD8,HLA DP,HLA DR in patients with both stromatic and ulcer herpetic keratitis,also CD10 in stromatic herpetic keratitis, were higher than in normal people. And the expression rate of CD9, CD45RA in patients with both stromatic and ulcer herpetic keratitis, also HLA ABC in ulcer herpetic keratitis,were lower than in normal people. The other epitopes in patients with both stromatic and ulcer herpetic keratitis had no difference with normal people. The expression rate of CD10,CD59,CD71 in patients with stromatic herpetic keratitis were higher than those in patients with ulcer herpetic keratitis. There were no difference in the other epitopes between the stromatic and the ulcer herpetic keratitis group. These results suggest that the immune disorder and autoimmunity may play an important role in the occurrence and clinical types of herpetic keratitis. The strong activating capacity for immunocyte were showed in individual with parenchymatous herpetic keratitis and the insufficient activating capacity for immunocyte were showed in individual with ulcer herpetic keratitis.